WinLTP
Version 2.30
Leading-Edge Features
Special Features
Special Analysis Features
Ordinary Features
Additional Features
Population Spike Measurement
 

There are several analysis programs that can measure Population Spike Area, Amplitude and Latency. However, to our  knowledge, WinLTP is the only acquisition program (other than proprietary software for MEA systems) that can do online Population Spike Area, Amplitude and Latency analysis.

The PopSpike Amplitude is calculated as the amplitude from the PopSpike peak to the intersection with an interpolated tangent dotted line drawn between the pre-PopSpike peak to the post-PopSpike peak (shown be the solid vertical line in the figure below). PopSpike Latency is calculated as the time between the occurrence of stimulus pulse and the PopSpike peak. PopSpike Area is the area between the tangent line and the waveform underneath. 

For more information see Section 4.11.9 in the on-line WinLTP Manual.

 

Detection of Population Spike Amplitude, Area and Latency. Detection occurs between the left and right brackets on the waveform. The solid vertical line is the Population Spike Amplitude, and the time between the stimulus pulse and the solid vertical line is the Population Spike Latency.  The area between the tangent line and the waveform is the Population Spike Area.